Comparison of the Diagnostic Sensitivity of Nasopharyngeal and Nasal Swabs and Use of Viral Loads for the Molecular Diagnosis of Equine Herpesvirus-1 Infection
نویسندگان
چکیده
The nasopharyngeal swabbing technique for the molecular detection of equine herpesvirus-1 (EHV-1) is not well tolerated in horses; nasal swabbing represents a viable alternative. Nasal swabs seem to be diagnostically more sensitive than nasopharyngeal swabs for the detection of low EHV-1 loads in naturally exposed horses. With regard to EHV-1 viral-load signatures in blood and nasal secretions, differences between clinically affected and subclinically affected horses and between those in the febrile and neurological stages of the disease are present on nasal swabs. The finding of high viral loads in the nasal secretions of neurological horses confirms their importance as a potential source of contagion for other horses and highlights the need for imposition of strict biosecurity measures when faced with horses with suspected or confirmed neurological EHV-1 infection. Our results also support the need for development of a consensus on the use and interpretation of molecular diagnostic techniques in the evaluation of field cases of suspected EHV-1 infection. Authors’ address: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA 95616; e-mail: [email protected]. © 2007 AAEP.
منابع مشابه
Diagnostic sensitivity of nasopharyngeal and nasal swabs for the molecular detection of EHV-1.
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تاریخ انتشار 2007